Ontology highlight
ABSTRACT:
SUBMITTER: Price IR
PROVIDER: S-EPMC4703321 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Price Ian R IR Gaballa Ahmed A Ding Fang F Helmann John D JD Ke Ailong A
Molecular cell 20150301 6
Gene regulation in cis by riboswitches is prevalent in bacteria. The yybP-ykoY riboswitch family is quite widespread, yet its ligand and function remained unknown. Here, we characterize the Lactococcus lactis yybP-ykoY orphan riboswitch as a Mn(2+)-dependent transcription-ON riboswitch, with a ∼30-40 μM affinity for Mn(2+). We further determined its crystal structure at 2.7 Å to elucidate the metal sensing mechanism. The riboswitch resembles a hairpin, with two coaxially stacked helices tethered ...[more]