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New insights into functional regulation in MS-based drug profiling.

ABSTRACT: We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.

SUBMITTER: Carvalho AS

PROVIDER: S-EPMC4705526 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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New insights into functional regulation in MS-based drug profiling.

Carvalho Ana Sofia AS Molina Henrik H Matthiesen Rune R

Scientific reports 20160108

We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables im ...[more]

PMID: 26743025

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