Dealing with the genetic load in bacterial synthetic biology circuits: convergences with the Ohm's law.

ABSTRACT: Synthetic biology seeks to envision living cells as a matter of engineering. However, increasing evidence suggests that the genetic load imposed by the incorporation of synthetic devices in a living organism introduces a sort of unpredictability in the design process. As a result, individual part characterization is not enough to predict the behavior of designed circuits and thus, a costly trial-error process is eventually required. In this work, we provide a new theoretical framework for the predictive treatment of the genetic load. We mathematically and experimentally demonstrate that dependences among genes follow a quantitatively predictable behavior. Our theory predicts the observed reduction of the expression of a given synthetic gene when an extra genetic load is introduced in the c

SUBMITTER: Carbonell-Ballestero M 

PROVIDER: S-EPMC4705654 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature