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Quality of histone modification antibodies undermines chromatin biology research.

ABSTRACT: Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.

SUBMITTER: Kungulovski G 

PROVIDER: S-EPMC4706057 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Quality of histone modification antibodies undermines chromatin biology research.

Kungulovski Goran G   Jeltsch Albert A  

F1000Research 20151028

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would  ...[more]