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ABSTRACT:
SUBMITTER: Atreya HS
PROVIDER: S-EPMC470728 | biostudies-literature | 2004 Jun
REPOSITORIES: biostudies-literature
Atreya Hanudatta S HS Szyperski Thomas T
Proceedings of the National Academy of Sciences of the United States of America 20040621 26
A G-matrix Fourier transform (GFT) NMR spectroscopy-based strategy for resonance assignment of proteins is described. Each of the GFT NMR experiments presented here rapidly affords four-, five-, or six-dimensional spectral information in combination with precise measurements of chemical shifts. The resulting high information content enables one to obtain nearly complete assignments by using only four NMR experiments. For the backbone amide proton detected "out-and-back" experiments, data collect ...[more]