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High-density self-contained microfluidic KOALA kits for use by everyone.

ABSTRACT: Cell-based assays are essential tools used by research labs in a wide range of fields, including cell biology, toxicology, and natural product discovery labs. However, in some situations, the need for cell-based assays does not justify the costs of maintaining cell culture facilities and retaining skilled staff. The kit-on-a-lid assay (KOALA) technology enables accessible low-cost and prepackageable microfluidic platforms that can be operated with minimal infrastructure or training. Here, we demonstrate and characterize high-density KOALA methods for high-throughput applications, achieving an assay density comparable to that of a 384-well plate and usability by hand with no liquid-handling equipment. We show the potential for high-content screening and complex assays such as quantitative immunochemistry assays requiring multiple steps and reagents.

SUBMITTER: Guckenberger DJ

PROVIDER: S-EPMC4707655 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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High-density self-contained microfluidic KOALA kits for use by everyone.

Guckenberger David J DJ Berthier Erwin E Beebe David J DJ

Journal of laboratory automation 20141125 2

Cell-based assays are essential tools used by research labs in a wide range of fields, including cell biology, toxicology, and natural product discovery labs. However, in some situations, the need for cell-based assays does not justify the costs of maintaining cell culture facilities and retaining skilled staff. The kit-on-a-lid assay (KOALA) technology enables accessible low-cost and prepackageable microfluidic platforms that can be operated with minimal infrastructure or training. Here, we dem ...[more]

PMID: 25424385

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