Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics.
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ABSTRACT:
SUBMITTER: Strzelecka D
PROVIDER: S-EPMC4708063 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
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Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics.
Strzelecka Dominika D Holman Stephen W SW Eyers Claire E CE
International journal of mass spectrometry 20151101
Dimethyl sulfoxide (DMSO) has been advocated as a beneficial additive to electrospray solvents for peptide analysis due to the improved ionisation efficiency conferred. Previous reports have shown that the resultant improvements in peptide ion signal intensities are non-uniform. As a result, it was hypothesised that inclusion of DMSO in electrospray solvents could be detrimental to the outcome of intensity-based label-free absolute quantification approaches, specifically the top 3 method. The ef ...[more]
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