Ontology highlight
ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC4708984 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Wang Hui H Yang Lian L Ping Yanhai Y Bai Yingguo Y Luo Huiying H Huang Huoqing H Yao Bin B
PloS one 20160111 1
The neutral protease has high potential for industrial applications, and attempts to improve enzyme expression level have important application values. In the present study, a neutral protease-encoding gene, Banpr, was cloned from Bacillus amyloliquefaciens strain K11, and a genetic manipulation method specific for this difficult-to-transform strain was developed for the high-level expression of neutral protease. The recombinant plasmid pUB110-Banpr was constructed in Bacillus subtilis strain WB ...[more]