Ontology highlight
ABSTRACT:
SUBMITTER: Du HX
PROVIDER: S-EPMC4709189 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Du Hao-Xing HX Xiao Wen-Hai WH Wang Ying Y Zhou Xiao X Zhang Yu Y Liu Duo D Yuan Ying-Jin YJ
PloS one 20160111 1
Campesterol is an important precursor for many sterol drugs, e.g. progesterone and hydrocortisone. In order to produce campesterol in Yarrowia lipolytica, C-22 desaturase encoding gene ERG5 was disrupted and the heterologous 7-dehydrocholesterol reductase (DHCR7) encoding gene was constitutively expressed. The codon-optimized DHCR7 from Rallus norvegicus, Oryza saliva and Xenapus laevis were explored and the strain with the gene DHCR7 from X. laevis achieved the highest titer of campesterol due ...[more]