Parallel Mapping of Antibiotic Resistance Alleles in Escherichia coli.
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ABSTRACT:
SUBMITTER: Weiss SJ
PROVIDER: S-EPMC4714920 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
Parallel Mapping of Antibiotic Resistance Alleles in Escherichia coli.
Weiss Sophie J SJ Mansell Thomas J TJ Mortazavi Pooneh P Knight Rob R Gill Ryan T RT
PloS one 20160115 1
Chemical genomics expands our understanding of microbial tolerance to inhibitory chemicals, but its scope is often limited by the throughput of genome-scale library construction and genotype-phenotype mapping. Here we report a method for rapid, parallel, and deep characterization of the response to antibiotics in Escherichia coli using a barcoded genome-scale library, next-generation sequencing, and streamlined bioinformatics software. The method provides quantitative growth data (over 200,000 m ...[more]
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