Ontology highlight
ABSTRACT:
SUBMITTER: Slaymaker IM
PROVIDER: S-EPMC4714946 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Slaymaker Ian M IM Gao Linyi L Zetsche Bernd B Scott David A DA Yan Winston X WX Zhang Feng F
Science (New York, N.Y.) 20151201 6268
The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing. Here, we use structure-guided protein engineering to improve the specificity of Streptococcus pyogenes ...[more]