Combining a Nitrogenase Scaffold and a Synthetic Compound into an Artificial Enzyme.
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ABSTRACT:
SUBMITTER: Tanifuji K
PROVIDER: S-EPMC4715667 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
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Combining a Nitrogenase Scaffold and a Synthetic Compound into an Artificial Enzyme.
Tanifuji Kazuki K Lee Chi Chung CC Ohki Yasuhiro Y Tatsumi Kazuyuki K Hu Yilin Y Ribbe Markus W MW
Angewandte Chemie (International ed. in English) 20151016 47
Nitrogenase catalyzes substrate reduction at its cofactor center ([(Cit)MoFe7S9C](n-); designated M-cluster). Here, we report the formation of an artificial, nitrogenase-mimicking enzyme upon insertion of a synthetic model complex ([Fe6S9(SEt)2](4-); designated Fe6(RHH)) into the catalytic component of nitrogenase (designated NifDK(apo)). Two Fe6(RHH) clusters were inserted into NifDK(apo), rendering the conformation of the resultant protein (designated NifDK(Fe)) similar to the one upon inserti ...[more]
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