'Deadman' and 'Passcode' microbial kill switches for bacterial containment.
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ABSTRACT:
SUBMITTER: Chan CT
PROVIDER: S-EPMC4718764 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Publications
'Deadman' and 'Passcode' microbial kill switches for bacterial containment.
Chan Clement T Y CT Lee Jeong Wook JW Cameron D Ewen DE Bashor Caleb J CJ Collins James J JJ
Nature chemical biology 20151207 2
Biocontainment systems that couple environmental sensing with circuit-based control of cell viability could be used to prevent escape of genetically modified microbes into the environment. Here we present two engineered safeguard systems known as the 'Deadman' and 'Passcode' kill switches. The Deadman kill switch uses unbalanced reciprocal transcriptional repression to couple a specific input signal with cell survival. The Passcode kill switch uses a similar two-layered transcription design and ...[more]
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