Evolution. Systematic humanization of yeast genes reveals conserved functions and genetic modularity.
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ABSTRACT:
SUBMITTER: Kachroo AH
PROVIDER: S-EPMC4718922 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Publications
Evolution. Systematic humanization of yeast genes reveals conserved functions and genetic modularity.
Kachroo Aashiq H AH Laurent Jon M JM Yellman Christopher M CM Meyer Austin G AG Wilke Claus O CO Marcotte Edward M EM
Science (New York, N.Y.) 20150501 6237
To determine whether genes retain ancestral functions over a billion years of evolution and to identify principles of deep evolutionary divergence, we replaced 414 essential yeast genes with their human orthologs, assaying for complementation of lethal growth defects upon loss of the yeast genes. Nearly half (47%) of the yeast genes could be successfully humanized. Sequence similarity and expression only partly predicted replaceability. Instead, replaceability depended strongly on gene modules: ...[more]
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