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Introducing MINA--The Molecularly Imprinted Nanoparticle Assay.

ABSTRACT: A new ELISA- (enzyme-linked immunosorbent assay)-like assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic core and which are synthesized by using a solid-phase approach can simultaneously act as recognition/signaling elements, and be used with minimal modifications to standard assay protocols. This assay provides a new route towards replacement of unstable biomolecules in immunoassays.

SUBMITTER: Shutov RV

PROVIDER: S-EPMC4719181 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Introducing MINA--The Molecularly Imprinted Nanoparticle Assay.

Shutov Roman V RV Guerreiro Antonio A Moczko Ewa E de Vargas-Sansalvador Isabel Perez IP Chianella Iva I Whitcombe Michael J MJ Piletsky Sergey A SA

Small (Weinheim an der Bergstrasse, Germany) 20140206 6

A new ELISA- (enzyme-linked immunosorbent assay)-like assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic core and which are synthesized by using a solid-phase approach can simultaneously act as recognition/signaling elements, and be used with minimal modifications to standard assay protocols. This assay provides a new route towards replacement of unstable biomolecules in immun ...[more]

PMID: 24504940

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