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Effect of extracorporeal bioartificial liver support system on fulminant hepatic failure rabbits.

ABSTRACT: AIM:To evaluate the possibility of using cultured human hepatocytes as a bridge between bioartificial liver and liver transplantation.METHODS:In this experiment,the efficacy of extracorporeal bioartificial liver support system (EBLSS) consisting of spheriodal human liver cells and cultured hepatocytes supernatant was assessed in vivo using galactosamine induced rabbit model of fulminant hepatic failure.RESULTS:There was no difference of survival between the two groups of rabbits, but in the supported rabbits serum alanine aminotransferase, total bilirubin and creatinine were significantly lower and hepatocyte necrosis was markedly milder than those in control animals. In addition, a good viability of human liver cells was noted after the experiment.CONCLUSION:EBLSS plays a biologic role in maintaining and compensating the function of the liver.

SUBMITTER: Wang YJ

PROVIDER: S-EPMC4723496 | biostudies-literature | 2000 Apr

REPOSITORIES: biostudies-literature

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Effect of extracorporeal bioartificial liver support system on fulminant hepatic failure rabbits.

Wang Ying-Jie YJ Li Meng-Dong MD Wang Yu-Ming YM Chen Guo-Zheng GZ Lu Guo-Dong GD Tan Zao-Xia ZX

World journal of gastroenterology 20000401 2

AIM:To evaluate the possibility of using cultured human hepatocytes as a bridge between bioartificial liver and liver transplantation.METHODS:In this experiment,the efficacy of extracorporeal bioartificial liver support system (EBLSS) consisting of spheriodal human liver cells and cultured hepatocytes supernatant was assessed in vivo using galactosamine induced rabbit model of fulminant hepatic failure.RESULTS:There was no difference of survival between the two groups of rabbits, but in the supp ...[more]

PMID: 11819568

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Project description:ImportanceAcute liver failure (ALF) carries significant morbidity and mortality, for both pediatric and adult patients. Albumin dialysis via the molecular adsorbent recirculating system (MARS) is a form of extracorporeal liver support (ELS) that can reduce hepatic encephalopathy (HE), a main driver of mortality in ALF. However, data on MARS and its benefit on mortality have been inconsistent.ObjectivesWe sought to report our experiences and patient outcomes from the first 2 years of operation of a new ELS program, within an established pediatric liver transplantation center.Design setting and participantsRetrospective review of outcomes in pediatric and adult patients treated with MARS therapy for ALF, from 2021 to 2022.Main outcomes and measuresOutcomes included reduction in HE and biochemical markers of ALF after MARS therapy, survival, and transplant-free survival. Comparisons were made via Wilcoxon signed-rank test.ResultsFive pediatric and two adult patients underwent MARS for ALF. Ages ranged from 2 to 29 years. Overall, 21 MARS runs were performed (median 3 runs per patient, 12.4 hr per run [interquartile range, IQR 10.1-17]). Overall survival was 85.7%, and transplant-free survival was 71.4%. There was a statistically significant reduction in HE score with MARS therapy (median 3 [IQR 3-4] to 1 [IQR 0-1], p = 0.03), and in ALF biomarkers including ammonia (256 µL/dL [195-265] to 75 µL/dL [58-101], p = 0.02), aspartate aminotransferase (6,362 U/L [920-8,305] to 212 U/L [72-431], p = 0.02), alanine aminotransferase (8,362 U/L [3,866-9,189] to 953 U/L [437-1,351], p = 0.02), and international normalized ratio (4.5 [3.3-6.7] to 1.3 [1.2-1.4], p = 0.02).Conclusions and relevanceMARS therapy for ALF was well tolerated by both pediatric and adult patients, and resulted in significant improvement in clinical and biochemical parameters. We demonstrated encouraging overall and transplant-free survival, suggesting that early initiation of MARS with relatively long and frequent cycle times may be of significant benefit to ALF patients, and is worthy of additional study in larger cohorts.

Dataset Information

Effect of extracorporeal bioartificial liver support system on fulminant hepatic failure rabbits.

Publications

Effect of extracorporeal bioartificial liver support system on fulminant hepatic failure rabbits.

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