Ontology highlight
ABSTRACT:
SUBMITTER: Taverna D
PROVIDER: S-EPMC4724279 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Taverna Domenico D Pollins Alonda C AC Nanney Lillian B LB Sindona Giovanni G Caprioli Richard M RM
Experimental dermatology 20151123 2
Herein we present a simple, reproducible and versatile approach for in situ protein digestion and identification on formalin-fixed and paraffin-embedded (FFPE) tissues. This adaptation is based on the use of an enzyme delivery platform (hydrogel discs) that can be positioned on the surface of a tissue section. By simultaneous deposition of multiple hydrogels over select regions of interest within the same tissue section, multiple peptide extracts can be obtained from discrete histological areas. ...[more]