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Longitudinal Survey of Microbiota in Hospitalized Preterm Very-Low-Birth-Weight Infants.


ABSTRACT: The aim of the present study was to examine the changes in bacteria in hospitalized preterm infants during the first month of life.Rectal swabs were collected daily from 12 preterm infants. DNA was extracted from swabs from day of birth and weekly thereafter. Bacterial taxa were identified with next generation sequencing using universal bacterial primers targeted at the 16S ribosomal DNA on a 454 Roche titanium platform. Sequences were clustered into operational taxonomic units, and taxonomy was assigned against the Greengenes databank using Quantitative Insights Into Microbial Ecology version 1.4. Quantitative polymerase chain reaction was used to determine the abundance of Bifidobacterium spp. Functional assessment of the microbiome was performed with Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).Average birth weight and gestational age were 1055 g and 28 weeks, respectively. There were 6 to 35 different bacterial families identified in the day-of-birth samples, unrelated to the mode of delivery. Richness decreased through hospitalization (week 1, 16.9?±?7.7 vs weeks 3-5, 10.7?±?3.4, P?

SUBMITTER: Patel AL 

PROVIDER: S-EPMC4724288 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Longitudinal Survey of Microbiota in Hospitalized Preterm Very-Low-Birth-Weight Infants.

Patel Aloka L AL   Mutlu Ece A EA   Sun Yan Y   Koenig Lars L   Green Stefan S   Jakubowicz Andrew A   Mryan Janet J   Engen Phillip P   Fogg Louis L   Chen Andrea L AL   Pombar Xavier X   Meier Paula P PP   Keshavarzian Ali A  

Journal of pediatric gastroenterology and nutrition 20160201 2


<h4>Objectives</h4>The aim of the present study was to examine the changes in bacteria in hospitalized preterm infants during the first month of life.<h4>Methods</h4>Rectal swabs were collected daily from 12 preterm infants. DNA was extracted from swabs from day of birth and weekly thereafter. Bacterial taxa were identified with next generation sequencing using universal bacterial primers targeted at the 16S ribosomal DNA on a 454 Roche titanium platform. Sequences were clustered into operationa  ...[more]

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