Surface-to-volume ratio mapping of tumor microstructure using oscillating gradient diffusion weighted imaging.

ABSTRACT:

Purpose

To disentangle the free diffusivity (D0 ) and cellular membrane restrictions, by means of their surface-to-volume ratio (S/V), using the frequency-dependence of the diffusion coefficient D(ω), measured in brain tumors in the short diffusion-time regime using oscillating gradients (OGSE).

Methods

In vivo and ex vivo OGSE experiments were performed on mice bearing the GL261 murine glioma model (n = 10) to identify the relevant time/frequency (t/ω) domain where D(ω) linearly decreases with ω(-1/2) . Parametric maps (S/V, D0 ) are compared with conventional DWI metrics. The impact of frequency range and temperature (20°C versus 37°C) on S/V and D0 is investigated ex vivo.

Results

The validity of the short diffusion-time regime is demonstrated in vivo and ex vivo.

SUBMITTER: Reynaud O 

PROVIDER: S-EPMC4724565 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature