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A Two-Photon Fluorescent Probe for Lysosomal Thiols in Live Cells and Tissues.

ABSTRACT: Lysosome-specific fluorescent probes are exclusive to elucidate the functions of lysosomal thiols. Moreover, two-photon microscopy offers advantages of less phototoxicity, better three dimensional spatial localization, deeper penetration depth and lower self-absorption. However, such fluorescent probes for thiols are still rare. In this work, an efficient two-photon fluorophore 1,8-naphthalimide-based probe conjugating a 2,4-dinitrobenzenesulfonyl chloride and morpholine was designed and synthesized, which exhibited high selectivity and sensitivity towards lysosomal thiols by turn-on fluorescence method quantitatively and was successfully applied to the imaging of thiols in live cells and tissues by two-photon microscopy.

SUBMITTER: Fan J

PROVIDER: S-EPMC4726187 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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