Development of an Extracorporeal Perfusion Device for Small Animal Free Flaps.

ABSTRACT:

Background

Extracorporeal perfusion (ECP) might prolong the vital storage capabilities of composite free flaps, potentially opening a wide range of clinical applications. Aim of the study was the development a validated low-cost extracorporeal perfusion model for further research in small animal free flaps.

Methods

After establishing optimal perfusion settings, a specially designed extracorporeal perfusion system was evaluated during 8-hour perfusion of rat epigastric flaps followed by microvascular free flap transfer. Controls comprised sham-operation, ischemia and in vivo perfusion. Flaps and perfusate (diluted blood) were closely monitored by blood gas analysis, combined laser Doppler flowmetry and remission spectroscopy and Indocyanine-Green angiography. Evaluations were

SUBMITTER: Fichter AM 

PROVIDER: S-EPMC4726627 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature