Dataset Information

Surface hydrophobicity of slippery zones in the pitchers of two Nepenthes species and a hybrid.

ABSTRACT: To investigate the hydrophobicity of slippery zones, static contact angle measurement and microstructure observation of slippery surfaces from two Nepenthes species and a hybrid were conducted. Marginally different static contact angles were observed, as the smallest (133.83°) and greatest (143.63°) values were recorded for the N. alata and N. miranda respectively, and the median value (140.40°) was presented for the N. khasiana. The slippery zones under investigation exhibited rather similar surface morphologies, but different structural dimensions. These findings probably suggest that the geometrical dimensions of surface architecture exert primary effects on differences in the hydrophobicity of the slippery zone. Based on the Wenzel and Cassie-Baxter equations, models were proposed to analyze the manner in which geometrical dimensions affect the hydrophobicity of the slippery surfaces. The results of our analysis demonstrated that the different structural dimensions of lunate cells and wax platelets make the slippery zones present different real area of the rough surface and thereby generate somewhat distinguishable hydrophobicity. The results support a supplementary interpretation of surface hydrophobicity in plant leaves, and provide a theoretical foundation for developing bioinspired materials with hydrophobic properties and self-cleaning abilities.

SUBMITTER: Wang L

PROVIDER: S-EPMC4728604 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Surface hydrophobicity of slippery zones in the pitchers of two Nepenthes species and a hybrid.

Wang Lixin L Zhou Qiang Q

Scientific reports 20160127

To investigate the hydrophobicity of slippery zones, static contact angle measurement and microstructure observation of slippery surfaces from two Nepenthes species and a hybrid were conducted. Marginally different static contact angles were observed, as the smallest (133.83°) and greatest (143.63°) values were recorded for the N. alata and N. miranda respectively, and the median value (140.40°) was presented for the N. khasiana. The slippery zones under investigation exhibited rather similar su ...[more]

PMID: 26813707

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Project description:Hybridization is key to the evolution and diversity of plants in nature. Nepenthaceae comprises a family of diverse tropical carnivorous pitcher plant species with extensive hybridization. However, there is no study to date on the metabolite expression of hybrids in this family. We performed a non-targeted metabolomics analysis of the pitchers of two Nepenthes species with different dietary habits, namely, the semi-detritivorous N. ampullaria and carnivorous N. rafflesiana with their hybrid (N. × hookeriana) for a comparative study. The whole-pitcher samples were extracted in methanol:chloroform:water (3:1:1) via sonication-assisted extraction and analyzed using ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) followed by data analysis to profile chemical compositions. A total of 1,441 metabolite features were profiled from the three species in which 43.3% of features in the hybrid samples were not found in either of its parents. The partial least squares discriminant analysis (PLS-DA) found 324 metabolite features with variable in projection (VIP) values greater than one in which 55 features were statistically significant. This showed that the hybrid is closer to N. rafflesiana, which is consistent to the previous study on gene and protein expressions. A total of 105 metabolites were putatively identified with manual searches using public metabolite databases. Phenols were detected to be the most abundant secondary metabolites due to a high flavonoid content, especially in N. rafflesiana. The most abundant feature 476.3s:449.102 was found to be the most significant VIP for distinguishing between the three species as a chemical marker. This is the first study comparing metabolites in the carnivory organs of different Nepenthes species with comprehensive profiling and putative identification. The differential metabolite compositions in the pitchers of different species might have ecological implications with the hybrid showing intermediate phenotype between the parents as well as manifesting unique metabolites. However, there is no clear evidence of metabolites related to the differences in dietary habits between the hybrid and the two parent species.

Project description:Nepenthes is a genus comprising carnivorous tropical pitcher plants that have evolved trapping organs at the tip of their leaves for nutrient acquisition from insect trapping. Recent studies have applied proteomics approaches to identify proteins in the pitcher fluids for better understanding the carnivory mechanism, but protein identification is hindered by limited species-specific transcriptomes for Nepenthes. In this study, the proteomics informed by transcriptomics (PIT) approach was utilized to identify and compare proteins in the pitcher fluids of Nepenthes ampullaria, Nepenthes rafflesiana, and their hybrid Nepenthes × hookeriana through PacBio isoform sequencing (Iso-Seq) and liquid chromatography-mass spectrometry (LC-MS) proteomic profiling. We generated full-length transcriptomes from all three species of 80,791 consensus isoforms with an average length of 1,692 bp as a reference for protein identification. The comparative analysis found that transcripts and proteins identified in the hybrid N. × hookeriana were more resembling N. rafflesiana, both of which are insectivorous compared with omnivorous N. ampullaria that can derive nutrients from leaf litters. Previously reported hydrolytic proteins were detected, including proteases, glucanases, chitinases, phosphatases, nucleases, peroxidases, lipid transfer protein, thaumatin-like protein, pathogenesis-related protein, and disease resistance proteins. Many new proteins with diverse predicted functions were also identified, such as amylase, invertase, catalase, kinases, ligases, synthases, esterases, transferases, transporters, and transcription factors. Despite the discovery of a few unique enzymes in N. ampullaria, we found no strong evidence of adaptive evolution to produce endogenous enzymes for the breakdown of leaf litter. A more complete picture of digestive fluid protein composition in this study provides important insights on the molecular physiology of pitchers and carnivory mechanism of Nepenthes species with distinct dietary habits.

Dataset Information

Surface hydrophobicity of slippery zones in the pitchers of two Nepenthes species and a hybrid.

Publications

Surface hydrophobicity of slippery zones in the pitchers of two Nepenthes species and a hybrid.

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