A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery.
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ABSTRACT:
SUBMITTER: Oba M
PROVIDER: S-EPMC4728608 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
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A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery.
Oba Makoto M Kato Takuma T Furukawa Kaori K Tanaka Masakazu M
Scientific reports 20160127
A peptide composed of lysine with a guanidinylethyl (GEt) amine structure in the side chain [Lys(GEt)] was developed as a cell-penetrating peptide directed to plasmid DNA (pDNA) delivery. The GEt amine adopted a diprotonated form at neutral pH, which may have led to the more efficient cellular uptake of a Lys(GEt)-peptide than an arginine-peptide at a low concentration. Lys(GEt)-peptide/pDNA complexes showed the highest transfection efficiency due to efficient endosomal escape without any cytoto ...[more]
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