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Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.

ABSTRACT: We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.

SUBMITTER: Liv N

PROVIDER: S-EPMC4729641 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.

Liv Nalan N van Oosten Slingeland Daan S B DS Baudoin Jean-Pierre JP Kruit Pieter P Piston David W DW Hoogenboom Jacob P JP

ACS nano 20151208 1

We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on ...[more]

PMID: 26580231

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