In vitro Biochemical Characterization of All Barley Endosperm Starch Synthases.

ABSTRACT: Starch is the main storage polysaccharide in cereals and the major source of calories in the human diet. It is synthesized by a panel of enzymes including five classes of starch synthases (SSs). While the overall starch synthase (SS) reaction is known, the functional differences between the five SS classes are poorly understood. Much of our knowledge comes from analyzing mutant plants with altered SS activities, but the resulting data are often difficult to interpret as a result of pleitropic effects, competition between enzymes, overlaps in enzyme activity and disruption of multi-enzyme complexes. Here we provide a detailed biochemical study of the activity of all five classes of SSs in barley endosperm. Each enzyme was produced recombinantly in E. coli and the properties and modes of act

SUBMITTER: Cuesta-Seijo JA 

PROVIDER: S-EPMC4730117 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature