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Characteristics of chlamydia-like organisms pathogenic to fish.

ABSTRACT: Bacteria from the Chlamydiales order have been long known, especially as pathogenic bacteria to humans and many animal species, principally including birds and mammals. But for slightly over 20 years, they have been identified in the aquatic environment as endosymbionts of amoebas and sea worms. For several years, they have also been recorded as a cause of diseases among fish, causing respiratory system infections in the form of epitheliocystis of the gill. At present, 11 chlamydia-like organisms pathogenic to fish have been described, including nine new ones, classified into six families, four of which are already known (Parachlamydiaceae, Rhabdochlamydiaceae, Candidatus Parilichlamydiaceae, Candidatus Clavichlamydiaceae) and two newly created families, namely Candidatus Actinochlamydiaceae and Candidatus Parilichlamydiaceae. This paper characterises 11 chlamydia-like organisms, as well as seven isolates not classified into families, which are pathogenic to fish, presenting their genetical properties allowing for their classification, as well as morphological properties and diseases caused.

SUBMITTER: Pawlikowska-Warych M

PROVIDER: S-EPMC4731428 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Characteristics of chlamydia-like organisms pathogenic to fish.

Pawlikowska-Warych Małgorzata M Deptuła Wiesław W

Journal of applied genetics 20150710 1

Bacteria from the Chlamydiales order have been long known, especially as pathogenic bacteria to humans and many animal species, principally including birds and mammals. But for slightly over 20 years, they have been identified in the aquatic environment as endosymbionts of amoebas and sea worms. For several years, they have also been recorded as a cause of diseases among fish, causing respiratory system infections in the form of epitheliocystis of the gill. At present, 11 chlamydia-like organism ...[more]

PMID: 26160214

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Project description:Infectious diseases remain as an important public health threat to humans. Research into pathogens can be useful for planning an organized response to infectious diseases. Bibliometric analysis is an important method for scientific research and assessing capability. In this study, 100 pathogens of public health importance were selected, and the distributions of annual, national, institutional, and journal publications on Science Citation Index (SCI) journals were statistically analyzed. The United States of America ranked the first in terms of the number of relevant studies published. China attaches great importance to the prevention and control of infectious diseases, but still needs to improve in the following areas: for example, insufficient SCI reports on particular pathogens, institutions SCI reports on pathogens lower than world top pathogen research institutions, and lack of influential international pathogen-related journals. Scientific literature databases are important tools for science-of-science analysis. The findings of this study shed light on the hot spots and the ignored spots in pathogen researches, and thus would be useful for drawing a national and institutional research plan. Highlights • Scientific question• Scientific literature databases are important tools for science-of-science analysis, which can be referred as Science of the Science of biosafety/biosecurity (SSBS) in the field of biosafety and biosecurity.• Evidence before this study• In this study, we conducted a comprehensive literature analysis of pathogens, including health-threatening pathogens (HTPs) and biodefense-associated pathogens (BDAPs).• New findings• Our new findings indicated that some literature on pathogens, particularly BDAPs, showed a decrease followed by an increase. The total number of Science Citation Index reports on pathogens in China is the second highest in the world, but it is significantly behind the number of published studies in the United States. Particularly, SCI reports on BDAPs in China is insufficient, specifically at the university level.• Significance of this study• The findings of this study highlight the hot spots and ignored spots in pathogen research; therefore, can be helpful on drawing a national and institutional research plan.

Project description:Chlamydia-like organisms (CLOs) are recently identified members of the Chlamydiales order. CLOs share intracellular lifestyles and biphasic developmental cycles, and they have been detected in environmental samples as well as in various hosts such as amoebae and arthropods. In this study, we screened bat feces for the presence of CLOs by molecular analysis. Using pan-Chlamydiales PCR targeting the 16S rRNA gene, Chlamydiales DNA was detected in 54% of the specimens. PCR amplification, sequencing, and phylogenetic analysis of the 16S rRNA and 23S rRNA genes were used to classify positive specimens and infer their phylogenetic relationships. Most sequences matched best with Rhabdochlamydia species or uncultured Chlamydia sequences identified in ticks. Another set of sequences matched best with sequences of the Chlamydia genus or uncultured Chlamydiales from snakes. To gain evidence of whether CLOs in bat feces are merely diet borne, we analyzed insects trapped from the same location where the bats foraged. Interestingly, the CLO sequences resembling Rhabdochlamydia spp. were detected in insect material as well, but the other set of CLO sequences was not, suggesting that this set might not originate from prey. Thus, bats represent another potential host for Chlamydiales and could harbor novel, previously unidentified members of this order. IMPORTANCE:Several pathogenic viruses are known to colonize bats, and recent analyses indicate that bats are also reservoir hosts for bacterial genera. Chlamydia-like organisms (CLOs) have been detected in several animal species. CLOs have high 16S rRNA sequence similarity to Chlamydiaceae and exhibit similar intracellular lifestyles and biphasic developmental cycles. Our study describes the frequent occurrence of CLO DNA in bat feces, suggesting an expanding host species spectrum for the Chlamydiales As bats can acquire various infectious agents through their diet, prey insects were also studied. We identified CLO sequences in bats that matched best with sequences in prey insects but also CLO sequences not detected in prey insects. This suggests that a portion of CLO DNA present in bat feces is not prey borne. Furthermore, some sequences from bat droppings not originating from their diet might well represent novel, previously unidentified members of the Chlamydiales order.

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Characteristics of chlamydia-like organisms pathogenic to fish.

Publications

Characteristics of chlamydia-like organisms pathogenic to fish.

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