Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases.

ABSTRACT: The goal of this study was to define Na,K-ATPase ? and ? subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The ?1, ?3, and ?1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. ?1?1 and ?3?1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na(+) affinity respectively and the highest K(+) affinity. Mesenchymal cell isozymes possess an intermediate Na(+) affinity and a low K(+) affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K(+) concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the ?3?1 isozyme. Thus, the ?3?1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.

SUBMITTER: Baker Bechmann M 

PROVIDER: S-EPMC4731494 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature