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Characterization of arginine kinase, anovel allergen of dermatophagoides farinae (Der f 20).


ABSTRACT: OBJECTIVE:To characterize a novel allergen, the Dermatophagoides farinae-derived arginine kinase (Der f 20). METHODS:The protein of Der f 20 was synthesized by genetic engineering approaches. The allergenicity of Der f 20 was tested by enzyme-linked immunosorbent assay and an airway allergy mouse model. RESULTS:The Der f 20 gene was cloned andpresented in the Gene Bank with an accession number of AAP57094. The Der f 20 is an arginine kinase (AK), whichshowed a close relationship with D. pteronyssinus AK and Aleuroglyphusovatus AK. Western-blot and ELISA studies showed the IgE binding capacity of Der f 20 was 66.7% in the sera from 6 dust mite allergic patients. Immune inhibition assayresults showed the IgE cross-reactivity between Der f 20 and DME (Dust mite extract). Positive responses to Der f 20 were 41.2% as shown by skin prick tests in 17 DME-allergic patients. In vitro experimental results showed that Der f 20 induced Th2 cell differentiation and the expression of T cell Ig mucin domain molecule-4 (TIM4) in DCs. Conclusions; The Der f 20 protein is a novel subtype of thedust mite allergen.

SUBMITTER: Xing P 

PROVIDER: S-EPMC4731678 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Characterization of arginine kinase, anovel allergen of dermatophagoides farinae (Der f 20).

Xing Peng P   Yu Haiqiong H   Li Meng M   Xiao Xiaojun X   Jiang Congli C   Mo Lihua L   Zhang Min M   Yang Pingchang P   Liu Zhigang Z  

American journal of translational research 20151215 12


<h4>Objective</h4>To characterize a novel allergen, the Dermatophagoides farinae-derived arginine kinase (Der f 20).<h4>Methods</h4>The protein of Der f 20 was synthesized by genetic engineering approaches. The allergenicity of Der f 20 was tested by enzyme-linked immunosorbent assay and an airway allergy mouse model.<h4>Results</h4>The Der f 20 gene was cloned andpresented in the Gene Bank with an accession number of AAP57094. The Der f 20 is an arginine kinase (AK), whichshowed a close relatio  ...[more]

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