Ontology highlight
ABSTRACT:
SUBMITTER: Vladimirova A
PROVIDER: S-EPMC4732527 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Vladimirova Anna A Patskovsky Yury Y Fedorov Alexander A AA Bonanno Jeffrey B JB Fedorov Elena V EV Toro Rafael R Hillerich Brandan B Seidel Ronald D RD Richards Nigel G J NG Almo Steven C SC Raushel Frank M FM
Journal of the American Chemical Society 20160112 3
5-Carboxyvanillate decarboxylase (LigW) catalyzes the conversion of 5-carboxyvanillate to vanillate in the biochemical pathway for the degradation of lignin. This enzyme was shown to require Mn(2+) for catalytic activity and the kinetic constants for the decarboxylation of 5-carboxyvanillate by the enzymes from Sphingomonas paucimobilis SYK-6 (kcat = 2.2 s(-1) and kcat/Km = 4.0 × 10(4) M(-1) s(-1)) and Novosphingobium aromaticivorans (kcat = 27 s(-1) and kcat/Km = 1.1 × 10(5) M(-1) s(-1)) were d ...[more]