Ontology highlight
ABSTRACT:
SUBMITTER: Kotkowiak W
PROVIDER: S-EPMC4735469 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Kotkowiak Weronika W Pasternak Anna A Kierzek Ryszard R
PloS one 20160201 2
In this study, several RNA polymerases were used for the first time to examine the possibility of transcriptional incorporation of 5'-N-triphosphates of 5'-amino-5'-deoxyribonucleosides (5'NH NTPs). The T3, T7, Sp6 and T7 Y639F RNA polymerases were employed to show that the full-length transcript cannot be synthesized. The results suggest that the application of 5'NH NTPs could decrease transcription reaction rates. What is more, the modification of transcription conditions had no influence on t ...[more]