Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis.
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ABSTRACT:
SUBMITTER: Wang C
PROVIDER: S-EPMC4735651 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Publications
Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis.
Wang Chunyan C Bae Jin H JH Zhang David Yu DY
Nature communications 20160119
DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG° of DNA motifs at temperatures and buffer conditions of interest, with significantly better accuracy (6- to 14-fold lower s.e.) than prior methods. The equilibrium constant of a reaction with ...[more]
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