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The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.

ABSTRACT: Expression of mouse C-type lectin-like receptor 2 (CLEC-2) has been reported on circulating CD11b(high) Gr-1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC-2 expression failed to use CLEC-2-deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC-2-deficient adult mice using two independent approaches and employed two anti-mouse CLEC-2 antibody clones to investigate surface expression on hematopoietic cells from peripheral blood and secondary lymphoid organs. We rule out constitutive CLEC-2 expression on resting DCs and show that CLEC-2 is upregulated in response to LPS-induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC-2 and suggest that both circulating B lymphocytes and CD11b(high) Gr-1(high) myeloid cells lose CLEC-2 following entry into secondary lymphoid organs. These results have significant implications for our understanding of CLEC-2 physiological functions.

SUBMITTER: Lowe KL

PROVIDER: S-EPMC4737233 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.

Lowe Kate L KL Navarro-Núñez Leyre L Bénézech Cécile C Nayar Saba S Kingston Bethany L BL Nieswandt Bernhard B Barone Francesca F Watson Steve P SP Buckley Christopher D CD Desanti Guillaume E GE

European journal of immunology 20150812 9

Expression of mouse C-type lectin-like receptor 2 (CLEC-2) has been reported on circulating CD11b(high) Gr-1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC-2 expression failed to use CLEC-2-deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC-2-deficient adult mice using t ...[more]

PMID: 26173808

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Dataset Information

The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.

Publications

The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.

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