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IGF-loaded silicon and zinc doped brushite cement: physico-mechanical characterization and in vivo osteogenesis evaluation.

ABSTRACT: Dopants play critical roles in controlling the physical, mechanical, degradation kinetics, and in vivo properties of calcium phosphates. The aim of the present study was to evaluate the effects of silicon (Si) and zinc (Zn) dopants on the physico-mechanical and in vivo osteogenesis properties of brushite cements (BrCs) alone and in combination with insulin like growth factor 1 (IGF-1). Addition of 0.5 wt% Si did not alter the setting time, ?-TCP content, and compressive strength of BrCs significantly; however, 0.25 wt% Zn incorporation was accompanied by a significant decrease in mechanical strength from 4.78 ± 0.21 MPa for pure BrC to 3.78 ± 0.59 MPa and 3.28 ± 0.22 MPa for Zn-BrC and Si/Zn-BrC, respectively. The in vivo bone regeneration properties of doped BrCs alone and in combination with IGF-1 were assessed and compared using chronological radiography, histology, scanning electron microscopy and fluorochrome labeling at 2 and 4 months post implantation in a rabbit femoral defect model. Based on in vivo characterization focusing on osteogenesis and vasculogenesis, Si-BrC and Si/Zn-BrC showed the best performance followed by Zn-BrC and pure BrCs. Addition of IGF-1 further improved bone regeneration. Our findings confirm that addition of Si and/or Zn alters the physico-mechanical properties of BrCs and promotes the early stage in vivo osseointegration and bone remodeling properties.

SUBMITTER: Vahabzadeh S

PROVIDER: S-EPMC4739637 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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IGF-loaded silicon and zinc doped brushite cement: physico-mechanical characterization and in vivo osteogenesis evaluation.

Vahabzadeh Sahar S Bandyopadhyay Amit A Bose Susmita S Mandal Rakesh R Nandi Samit Kumar SK

Integrative biology : quantitative biosciences from nano to macro 20151104 12

Dopants play critical roles in controlling the physical, mechanical, degradation kinetics, and in vivo properties of calcium phosphates. The aim of the present study was to evaluate the effects of silicon (Si) and zinc (Zn) dopants on the physico-mechanical and in vivo osteogenesis properties of brushite cements (BrCs) alone and in combination with insulin like growth factor 1 (IGF-1). Addition of 0.5 wt% Si did not alter the setting time, β-TCP content, and compressive strength of BrCs signific ...[more]

PMID: 26530147

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Project description:Powder-liquid poly (methyl methacrylate) (PMMA) bone cements are widely utilized for augmentation of bone fractures and fixation of orthopedic implants. These cements typically have an abundance of beneficial qualities, however their lack of bioactivity allows for continued development. To enhance osseointegration and bioactivity, calcium phosphate cements prepared with hydroxyapatite, brushite or tricalcium phosphates have been introduced with rather unsuccessful results due to increased cement viscosity, poor handling and reduced mechanical performance. This has limited the use of such cements in applications requiring delivery through small cannulas and in load bearing. The goal of this study is to design an alternative cement system that can better accommodate calcium-phosphate additives while preserving cement rheological properties and performance. In the present work, a number of brushite-filled two-solution bone cements were prepared and characterized by studying their complex viscosity-versus-test frequency, extrusion stress, clumping tendency during injection through a syringe, extent of fill of a machined void in cortical bone analog specimens, and compressive strength. The addition of brushite into the two-solution cement formulations investigated did not affect the pseudoplastic behavior and handling properties of the materials as demonstrated by rheological experiments. Extrusion stress was observed to vary with brushite concentration with values lower or in the range of control PMMA-based cements. The materials were observed to completely fill pre-formed voids in bone analog specimens. Cement compressive strength was observed to decrease with increasing concentration of fillers; however, the materials exhibited high enough strength for consideration in load bearing applications. The results indicated that partially substituting the PMMA phase of the two-solution cement with brushite at a 40% by mass concentration provided the best combination of the properties investigated. This alternative material may find applications in systems requiring highly injectable and viscous cements such as in the treatment of spinal fractures and bone defects.

Dataset Information

IGF-loaded silicon and zinc doped brushite cement: physico-mechanical characterization and in vivo osteogenesis evaluation.

Publications

IGF-loaded silicon and zinc doped brushite cement: physico-mechanical characterization and in vivo osteogenesis evaluation.

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