2.9 A Resolution Cryo-EM 3D Reconstruction of Close-Packed Virus Particles.

ABSTRACT: Single-particle cryoelectron microscopy typically discards close-packed particle images as unusable data. Here, we report an image processing strategy and case study of obtaining near-atomic resolution 3D reconstructions from close-packed particles. Multiple independent de novo initial models were constructed to determine and cross-validate the particle parameters. The particles with consistent views were further refined including not only Euler angles and center positions but also defocus, astigmatism, beam tilt, and overall and anisotropic magnification. We demonstrated this strategy with a 2.9 Å resolution reconstruction of a 1.67 MDa virus-like particle of a circovirus, PCV2, recorded on 86 photographic films. The map resolution was further validated with a phase-randomization test and

SUBMITTER: Liu Z 

PROVIDER: S-EPMC4740195 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature