Dataset Information

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius.

ABSTRACT: The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971?kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding and noncoding gene models. The most pronounced change in gene expression during the life cycle occurs after feeding on human blood and included genes from the Wolbachia endosymbiont, which shows a simultaneous and coordinated host/commensal response to haematophagous activity. These data provide a rich genetic resource for mapping activity and density of C. lectularius across human hosts and cities, which can help track, manage and control bed bug infestations.

SUBMITTER: Rosenfeld JA

PROVIDER: S-EPMC4740774 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius.

Rosenfeld Jeffrey A JA Reeves Darryl D Brugler Mercer R MR Narechania Apurva A Simon Sabrina S Durrett Russell R Foox Jonathan J Shianna Kevin K Schatz Michael C MC Gandara Jorge J Afshinnekoo Ebrahim E Lam Ernest T ET Hastie Alex R AR Chan Saki S Cao Han H Saghbini Michael M Kentsis Alex A Planet Paul J PJ Kholodovych Vladyslav V Tessler Michael M Baker Richard R DeSalle Rob R Sorkin Louis N LN Kolokotronis Sergios-Orestis SO Siddall Mark E ME Amato George G Mason Christopher E CE

Nature communications 20160202

The common bed bug (Cimex lectularius) has been a persistent pest of humans for thousands of years, yet the genetic basis of the bed bug's basic biology and adaptation to dense human environments is largely unknown. Here we report the assembly, annotation and phylogenetic mapping of the 697.9-Mb Cimex lectularius genome, with an N50 of 971 kb, using both long and short read technologies. A RNA-seq time course across all five developmental stages and male and female adults generated 36,985 coding ...[more]

PMID: 26836631

Similar Datasets

Project description:BackgroundBed bugs (Cimex lectularius) are blood-feeding insects poised to become one of the major pests in households throughout the United States. Resistance of C. lectularius to insecticides/pesticides is one factor thought to be involved in its sudden resurgence. Despite its high-impact status, scant knowledge exists at the genomic level for C. lectularius. Hence, we subjected the C. lectularius transcriptome to 454 pyrosequencing in order to identify potential genes involved in pesticide resistance.Methodology and principal findingsUsing 454 pyrosequencing, we obtained a total of 216,419 reads with 79,596,412 bp, which were assembled into 35,646 expressed sequence tags (3902 contigs and 31744 singletons). Nearly 85.9% of the C. lectularius sequences showed similarity to insect sequences, but 44.8% of the deduced proteins of C. lectularius did not show similarity with sequences in the GenBank non-redundant database. KEGG analysis revealed putative members of several detoxification pathways involved in pesticide resistance. Lamprin domains, Protein Kinase domains, Protein Tyrosine Kinase domains and cytochrome P450 domains were among the top Pfam domains predicted for the C. lectularius sequences. An initial assessment of putative defense genes, including a cytochrome P450 and a glutathione-S-transferase (GST), revealed high transcript levels for the cytochrome P450 (CYP9) in pesticide-exposed versus pesticide-susceptible C. lectularius populations. A significant number of single nucleotide polymorphisms (296) and microsatellite loci (370) were predicted in the C. lectularius sequences. Furthermore, 59 putative sequences of Wolbachia were retrieved from the database.ConclusionsTo our knowledge this is the first study to elucidate the genetic makeup of C. lectularius. This pyrosequencing effort provides clues to the identification of potential detoxification genes involved in pesticide resistance of C. lectularius and lays the foundation for future functional genomics studies.

Dataset Information

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius.

Publications

Genome assembly and geospatial phylogenomics of the bed bug Cimex lectularius.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets