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RUNX1 haploinsufficiency results in granulocyte colony-stimulating factor hypersensitivity.


ABSTRACT: RUNX1/AML1 is among the most commonly mutated genes in human leukemia. Haploinsufficiency of RUNX1 causes familial platelet disorder with predisposition to myeloid malignancies (FPD/MM). However, the molecular mechanism of FPD/MM remains unknown. Here we show that murine Runx1(+/-) hematopoietic cells are hypersensitive to granulocyte colony-stimulating factor (G-CSF), leading to enhanced expansion and mobilization of stem/progenitor cells and myeloid differentiation block. Upon G-CSF stimulation, Runx1(+/-) cells exhibited a more pronounced phosphorylation of STAT3 as compared with Runx1(+/+) cells, which may be due to reduced expression of Pias3, a key negative regulator of STAT3 signaling, and reduced physical sequestration of STAT3 by RUNX1. Most importantly, blood cells from a FPD patient with RUNX1 mutation exhibited similar G-CSF hypersensitivity. Taken together, Runx1 haploinsufficiency appears to predispose FPD patients to MM by expanding the pool of stem/progenitor cells and blocking myeloid differentiation in response to G-CSF.

SUBMITTER: Chin DW 

PROVIDER: S-EPMC4742622 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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RUNX1 haploinsufficiency results in granulocyte colony-stimulating factor hypersensitivity.

Chin D W L DW   Sakurai M M   Nah G S S GS   Du L L   Jacob B B   Yokomizo T T   Matsumura T T   Suda T T   Huang G G   Fu X-Y XY   Ito Y Y   Nakajima H H   Osato M M  

Blood cancer journal 20160108


RUNX1/AML1 is among the most commonly mutated genes in human leukemia. Haploinsufficiency of RUNX1 causes familial platelet disorder with predisposition to myeloid malignancies (FPD/MM). However, the molecular mechanism of FPD/MM remains unknown. Here we show that murine Runx1(+/-) hematopoietic cells are hypersensitive to granulocyte colony-stimulating factor (G-CSF), leading to enhanced expansion and mobilization of stem/progenitor cells and myeloid differentiation block. Upon G-CSF stimulatio  ...[more]

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