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Maternal transcription of non-protein coding RNAs from the PWS-critical region rescues growth retardation in mice.

ABSTRACT: Prader-Willi syndrome (PWS) is a neurogenetic disorder caused by loss of paternally expressed genes on chromosome 15q11-q13. The PWS-critical region (PWScr) contains an array of non-protein coding IPW-A exons hosting intronic SNORD116 snoRNA genes. Deletion of PWScr is associated with PWS in humans and growth retardation in mice exhibiting ~15% postnatal lethality in C57BL/6 background. Here we analysed a knock-in mouse containing a 5'HPRT-LoxP-Neo(R) cassette (5'LoxP) inserted upstream of the PWScr. When the insertion was inherited maternally in a paternal PWScr-deletion mouse model (PWScr(p-/m5'LoxP)), we observed compensation of growth retardation and postnatal lethality. Genomic methylation pattern and expression of protein-coding genes remained unaltered at the PWS-locus of PWScr(p-/m5'LoxP) mice. Interestingly, ubiquitous Snord116 and IPW-A exon transcription from the originally silent maternal chromosome was detected. In situ hybridization indicated that PWScr(p-/m5'LoxP) mice expressed Snord116 in brain areas similar to wild type animals. Our results suggest that the lack of PWScr RNA expression in certain brain areas could be a primary cause of the growth retardation phenotype in mice. We propose that activation of disease-associated genes on imprinted regions could lead to general therapeutic strategies in associated diseases.

SUBMITTER: Rozhdestvensky TS

PROVIDER: S-EPMC4742849 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Maternal transcription of non-protein coding RNAs from the PWS-critical region rescues growth retardation in mice.

Rozhdestvensky Timofey S TS Robeck Thomas T Galiveti Chenna R CR Raabe Carsten A CA Seeger Birte B Wolters Anna A Gubar Leonid V LV Brosius Jürgen J Skryabin Boris V BV

Scientific reports 20160205

Prader-Willi syndrome (PWS) is a neurogenetic disorder caused by loss of paternally expressed genes on chromosome 15q11-q13. The PWS-critical region (PWScr) contains an array of non-protein coding IPW-A exons hosting intronic SNORD116 snoRNA genes. Deletion of PWScr is associated with PWS in humans and growth retardation in mice exhibiting ~15% postnatal lethality in C57BL/6 background. Here we analysed a knock-in mouse containing a 5'HPRT-LoxP-Neo(R) cassette (5'LoxP) inserted upstream of the P ...[more]

PMID: 26848093

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Dataset Information

Maternal transcription of non-protein coding RNAs from the PWS-critical region rescues growth retardation in mice.

Publications

Maternal transcription of non-protein coding RNAs from the PWS-critical region rescues growth retardation in mice.

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