Circulation of avian paramyxoviruses in wild birds of Kazakhstan in 2002-2013.

ABSTRACT:

Background

Screening wild birds for avian paramyxoviruses is of increasing importance. 6913 samples of tracheal and cloacal swabs were collected during 2002-2013 and tested to study the prevalence of APMVs in wild avifauna of Kazakhstan. As a result, 45 isolates were obtained during this period and their ecological niches and genetic relationships were defined.

Methods

Tracheal and cloacal samples from wild birds were collected using sterile swabs placed in viral transport medium and kept in liquid nitrogen until delivery to the laboratory. Samples were inoculated into 10-day-old embryonated chicken eggs and reverse transcription PCR (RT-PCR) assays were performed via a one-step protocol. The PCR products were sequenced and phylogenetic trees were constructed using the 'Neig

SUBMITTER: Karamendin K 

PROVIDER: S-EPMC4743248 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature