S-EPMC4743857 - Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus. - OmicsDI

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Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus.

ABSTRACT: Influenza A is a negative sense RNA virus that kills hundreds of thousands of humans each year. Base pairing in RNA is very favorable, but possibilities for RNA secondary structure of the influenza genomic RNA have not been investigated. This work presents the first experimentally-derived exploration of potential secondary structure in an influenza A naked (protein-free) genomic segment. Favorable folding regions are revealed by in vitro chemical structure mapping, thermodynamics, bioinformatics, and binding to isoenergetic microarrays of an entire natural sequence of the 875 nt segment 8 vRNA and of a smaller fragment. Segment 8 has thermodynamically stable and evolutionarily conserved RNA structure and encodes essential viral proteins NEP and NS1. This suggests that vRNA self-folding may generate helixes and loops that are important at one or more stages of the influenza life cycle.

SUBMITTER: Lenartowicz E

PROVIDER: S-EPMC4743857 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus.

Lenartowicz Elzbieta E Kesy Julita J Ruszkowska Agnieszka A Soszynska-Jozwiak Marta M Michalak Paula P Moss Walter N WN Turner Douglas H DH Kierzek Ryszard R Kierzek Elzbieta E

PloS one 20160205 2

Influenza A is a negative sense RNA virus that kills hundreds of thousands of humans each year. Base pairing in RNA is very favorable, but possibilities for RNA secondary structure of the influenza genomic RNA have not been investigated. This work presents the first experimentally-derived exploration of potential secondary structure in an influenza A naked (protein-free) genomic segment. Favorable folding regions are revealed by in vitro chemical structure mapping, thermodynamics, bioinformatics ...[more]

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