Ontology highlight
ABSTRACT:
SUBMITTER: Farino ZJ
PROVIDER: S-EPMC4743966 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Farino Zachary J ZJ Morgenstern Travis J TJ Vallaghe Julie J Gregor Nathalie N Donthamsetti Prashant P Harris Paul E PE Pierre Nicolas N Freyberg Robin R Charrier-Savournin Fabienne F Javitch Jonathan A JA Freyberg Zachary Z
PloS one 20160205 2
Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by w ...[more]