Ontology highlight
ABSTRACT:
SUBMITTER: Lippok S
PROVIDER: S-EPMC4744175 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Biophysical journal 20160201 3
Proteolysis of the multimeric blood coagulation protein von Willebrand Factor (VWF) by ADAMTS13 is crucial for prevention of microvascular thrombosis. ADAMTS13 cleaves VWF within the mechanosensitive A2 domain, which is believed to open under shear flow. In this study, we combine fluorescence correlation spectroscopy (FCS) and a microfluidic shear cell to monitor real-time kinetics of full-length VWF proteolysis as a function of shear stress. For comparison, we also measure the Michaelis-Menten ...[more]