Ontology highlight
ABSTRACT:
SUBMITTER: Kofler R
PROVIDER: S-EPMC4744716 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Kofler Robert R Nolte Viola V Schlötterer Christian C
Molecular ecology resources 20150609 1
Sequencing pools of individuals (Pool-Seq) is a cost-effective method to determine genome-wide allele frequency estimates. Given the importance of meta-analyses combining data sets, we determined the influence of different genomic library preparation protocols on the consistency of allele frequency estimates. We found that typically no more than 1% of the variation in allele frequency estimates could be attributed to differences in library preparation. Also read length had only a minor effect on ...[more]