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NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

ABSTRACT: Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C (NudC), an Hsp90 co-chaperone, is required for actin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates actin cytoskeleton and ciliogenesis by stabilizing cofilin 1.

SUBMITTER: Zhang C

PROVIDER: S-EPMC4746604 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Zhang Cheng C Zhang Wen W Lu Yi Y Yan Xiaoyi X Yan Xiumin X Zhu Xueliang X Liu Wei W Yang Yuehong Y Zhou Tianhua T

Cell research 20151225 2

Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C (NudC), an Hsp90 co-chaperone, is required for actin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. ...[more]

PMID: 26704451

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