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Enhanced electrostatic force microscopy reveals higher-order DNA looping mediated by the telomeric protein TRF2.


ABSTRACT: Shelterin protein TRF2 modulates telomere structures by promoting dsDNA compaction and T-loop formation. Advancement of our understanding of the mechanism underlying TRF2-mediated DNA compaction requires additional information regarding DNA paths in TRF2-DNA complexes. To uncover the location of DNA inside protein-DNA complexes, we recently developed the Dual-Resonance-frequency-Enhanced Electrostatic force Microscopy (DREEM) imaging technique. DREEM imaging shows that in contrast to chromatin with DNA wrapping around histones, large TRF2-DNA complexes (with volumes larger than TRF2 tetramers) compact DNA inside TRF2 with portions of folded DNA appearing at the edge of these complexes. Supporting coarse-grained molecular dynamics simulations uncover the structural requirement and sequential steps during TRF2-mediated DNA compaction and result in folded DNA structures with protruding DNA loops as seen in DREEM imaging. Revealing DNA paths in TRF2 complexes provides new mechanistic insights into structure-function relationships underlying telomere maintenance pathways.

SUBMITTER: Kaur P 

PROVIDER: S-EPMC4746636 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Enhanced electrostatic force microscopy reveals higher-order DNA looping mediated by the telomeric protein TRF2.

Kaur Parminder P   Wu Dong D   Lin Jiangguo J   Countryman Preston P   Bradford Kira C KC   Erie Dorothy A DA   Riehn Robert R   Opresko Patricia L PL   Wang Hong H  

Scientific reports 20160209


Shelterin protein TRF2 modulates telomere structures by promoting dsDNA compaction and T-loop formation. Advancement of our understanding of the mechanism underlying TRF2-mediated DNA compaction requires additional information regarding DNA paths in TRF2-DNA complexes. To uncover the location of DNA inside protein-DNA complexes, we recently developed the Dual-Resonance-frequency-Enhanced Electrostatic force Microscopy (DREEM) imaging technique. DREEM imaging shows that in contrast to chromatin w  ...[more]

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