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Product chain-length determination mechanism of Z,E-farnesyl diphosphate synthase.


ABSTRACT: cis-Prenyltransferases catalyze the consecutive condensation of isopentenyl diphosphate (IPP) with allylic prenyl diphosphates, producing Z,E-mixed prenyl diphosphate. The Mycobacterium tuberculosis Z,E-farnesyl diphosphate synthase Rv1086 catalyzes the condensation of one molecule of IPP with geranyl diphosphate to yield Z,E-farnesyl diphosphate and is classified as a short-chain cis-prenyltransferase. To elucidate the chain-length determination mechanism of the short-chain cis-prenyltransferase, we introduced some substitutive mutations at the characteristic amino acid residues of Rv1086. Among the mutants constructed, L84A showed a dramatic change of catalytic function to synthesize longer prenyl chain products than that of wild type, indicating that Leu84 of Rv1086 plays an important role in product chain-length determination. Mutagenesis at the corresponding residue of a medium-chain cis-prenyltransferase, Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase also resulted in the production of different prenyl chain length from the intrinsic product, suggesting that this position also plays an important role in product chain-length determination for medium-chain cis-prenyltransferases.

SUBMITTER: Noike M 

PROVIDER: S-EPMC4747032 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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Product chain-length determination mechanism of Z,E-farnesyl diphosphate synthase.

Noike Motoyoshi M   Ambo Takanori T   Kikuchi Sayaka S   Suzuki Toshihide T   Yamashita Satoshi S   Takahashi Seiji S   Kurokawa Hirofumi H   Mahapatra Sebabrata S   Crick Dean C DC   Koyama Tanetoshi T  

Biochemical and biophysical research communications 20080913 1


cis-Prenyltransferases catalyze the consecutive condensation of isopentenyl diphosphate (IPP) with allylic prenyl diphosphates, producing Z,E-mixed prenyl diphosphate. The Mycobacterium tuberculosis Z,E-farnesyl diphosphate synthase Rv1086 catalyzes the condensation of one molecule of IPP with geranyl diphosphate to yield Z,E-farnesyl diphosphate and is classified as a short-chain cis-prenyltransferase. To elucidate the chain-length determination mechanism of the short-chain cis-prenyltransferas  ...[more]

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