Unknown

Dataset Information

0

Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display.

ABSTRACT: Neisseria gonorrhoeae is one of the most prevalent sexually transmitted diseases worldwide with more than 100 million new infections per year. A lack of intense research over the last decades and increasing resistances to the recommended antibiotics call for a better understanding of gonococcal infection, fast diagnostics and therapeutic measures against N. gonorrhoeae. Therefore, the aim of this work was to identify novel immunogenic proteins as a first step to advance those unresolved problems. For the identification of immunogenic proteins, pHORF oligopeptide phage display libraries of the entire N. gonorrhoeae genome were constructed. Several immunogenic oligopeptides were identified using polyclonal rabbit antibodies against N. gonorrhoeae. Corresponding full-length proteins of the identified oligopeptides were expressed and their immunogenic character was verified by ELISA. The immunogenic character of six proteins was identified for the first time. Additional 13 proteins were verified as immunogenic proteins in N. gonorrhoeae.

SUBMITTER: Connor DO 

PROVIDER: S-EPMC4747489 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display.

Connor Daniel O DO   Zantow Jonas J   Hust Michael M   Bier Frank F FF   von Nickisch-Rosenegk Markus M  

PloS one 20160209 2

Neisseria gonorrhoeae is one of the most prevalent sexually transmitted diseases worldwide with more than 100 million new infections per year. A lack of intense research over the last decades and increasing resistances to the recommended antibiotics call for a better understanding of gonococcal infection, fast diagnostics and therapeutic measures against N. gonorrhoeae. Therefore, the aim of this work was to identify novel immunogenic proteins as a first step to advance those unresolved problems  ...[more]

Similar Datasets

Unknown Epitope determination of immunogenic proteins of Neisseria gonorrhoeae.
| S-EPMC5516995 | biostudies-literature
Unknown Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display.
| S-EPMC3423037 | biostudies-literature
Unknown Application of M13 phage display for identifying immunogenic proteins from tick (Ixodes scapularis) saliva.
| S-EPMC4449557 | biostudies-literature
Unknown Phage proteins are expressed on the surface of Neisseria gonorrhoeae and are potential vaccine candidates.
| S-EPMC6107182 | biostudies-literature
Unknown Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.
| S-EPMC7561177 | biostudies-literature
Unknown Efficient identification of phosphatidylserine-binding proteins by ORF phage display.
| S-EPMC2714054 | biostudies-literature
Unknown Identification of Novel Neisseria gonorrhoeae Lineages Harboring Resistance Plasmids in Coastal Kenya.
| S-EPMC6057544 | biostudies-literature
Unknown Novel lipoprotein expressed by Neisseria meningitidis but not by Neisseria gonorrhoeae.
| S-EPMC173202 | biostudies-other
Transcriptomics Transcriptomics of Neisseria gonorrhoeae
2016-05-24 | GSE73032 | GEO
Unknown The Neisseria gonorrhoeae Methionine Sulfoxide Reductase (MsrA/B) Is a Surface Exposed, Immunogenic, Vaccine Candidate.
| S-EPMC6372556 | biostudies-literature