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The diagnostic accuracy of the MTBDRplus and MTBDRsl assays for drug-resistant TB detection when performed on sputum and culture isolates.

ABSTRACT: Although molecular tests for drug-resistant TB perform well on culture isolates, their accuracy using clinical samples, particularly from TB and HIV-endemic settings, requires clarification. The MTBDRplus and MTBDRsl line probe assays were evaluated in 181 sputum samples and 270 isolates from patients with culture-confirmed drug-sensitive-TB, MDR-TB, or XDR-TB. Phenotypic culture-based testing was the reference standard. Using sputum, the sensitivities for resistance was 97.7%, 95.4%, 58.9%, 61.6% for rifampicin, isoniazid, ofloxacin, and amikacin, respectively, whereas the specificities were 91.8%, 89%, 100%, and 100%, respectively. MTBDRsl sensitivity differed in smear-positive vs. smear-negative samples (79.2% vs. 20%, p?

SUBMITTER: Tomasicchio M

PROVIDER: S-EPMC4748215 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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The diagnostic accuracy of the MTBDRplus and MTBDRsl assays for drug-resistant TB detection when performed on sputum and culture isolates.

Tomasicchio Michele M Theron Grant G Pietersen Elize E Streicher Elizabeth E Stanley-Josephs Danielle D van Helden Paul P Warren Rob R Dheda Keertan K

Scientific reports 20160210

Although molecular tests for drug-resistant TB perform well on culture isolates, their accuracy using clinical samples, particularly from TB and HIV-endemic settings, requires clarification. The MTBDRplus and MTBDRsl line probe assays were evaluated in 181 sputum samples and 270 isolates from patients with culture-confirmed drug-sensitive-TB, MDR-TB, or XDR-TB. Phenotypic culture-based testing was the reference standard. Using sputum, the sensitivities for resistance was 97.7%, 95.4%, 58.9%, 61. ...[more]

PMID: 26860462

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Diagnostic performance of oral swabs for non-sputum based TB diagnosis in a TB/HIV endemic setting.

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Performance of GenoType MTBDRsl assay for detection of second-line drugs and ethambutol resistance directly from sputum specimens of MDR-TB patients in Bangladesh.

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TB sputum metagenomics

Project description:Metagenomics of TB-associated sputum

| PRJEB7248 | ENA

Rats sniff out pulmonary tuberculosis from sputum: a diagnostic accuracy meta-analysis.

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| S-EPMC7820466 | biostudies-literature

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