Unknown

Dataset Information

0

MiR-126 Regulates Distinct Self-Renewal Outcomes in Normal and Malignant Hematopoietic Stem Cells.


ABSTRACT: To investigate miRNA function in human acute myeloid leukemia (AML) stem cells (LSC), we generated a prognostic LSC-associated miRNA signature derived from functionally validated subpopulations of AML samples. For one signature miRNA, miR-126, high bioactivity aggregated all in vivo patient sample LSC activity into a single sorted population, tightly coupling miR-126 expression to LSC function. Through functional studies, miR-126 was found to restrain cell cycle progression, prevent differentiation, and increase self-renewal of primary LSC in vivo. Compared with prior results showing miR-126 regulation of normal hematopoietic stem cell (HSC) cycling, these functional stem effects are opposite between LSC and HSC. Combined transcriptome and proteome analysis demonstrates that miR-126 targets the PI3K/AKT/MTOR signaling pathway, preserving LSC quiescence and promoting chemotherapy resistance.

SUBMITTER: Lechman ER 

PROVIDER: S-EPMC4749543 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications


To investigate miRNA function in human acute myeloid leukemia (AML) stem cells (LSC), we generated a prognostic LSC-associated miRNA signature derived from functionally validated subpopulations of AML samples. For one signature miRNA, miR-126, high bioactivity aggregated all in vivo patient sample LSC activity into a single sorted population, tightly coupling miR-126 expression to LSC function. Through functional studies, miR-126 was found to restrain cell cycle progression, prevent differentiat  ...[more]

Similar Datasets

| S-EPMC5551568 | biostudies-literature
2017-07-27 | GSE101958 | GEO
| PRJNA396018 | ENA
| S-EPMC6567521 | biostudies-literature
| S-EPMC4032599 | biostudies-literature
| S-EPMC4922705 | biostudies-other
| S-EPMC5939047 | biostudies-literature
| S-EPMC3889555 | biostudies-literature
| S-EPMC3832064 | biostudies-literature
| S-EPMC5260904 | biostudies-literature