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HCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles.

ABSTRACT: The influenza A virus polymerase associates with a number of cellular transcription-related factors, including the RNA polymerase II (RNAP II). We previously described that the cellular protein hCLE/C14orf166 interacts with and stimulates influenza virus polymerase as well as RNAP II activities. Here we show that, despite the considerable cellular shut-off observed in infected cells, which includes RNAP II degradation, hCLE protein levels increase throughout infection in a virus replication-dependent manner. Human and avian influenza viruses of various subtypes increase hCLE levels, but other RNA or DNA viruses do not. hCLE colocalises and interacts with viral ribonucleoproteins (vRNP) in the nucleus, as well as in the cytoplasm late in infection. Furthermore, biochemical analysis of purified virus particles and immunoelectron microscopy of infected cells show hCLE in virions, in close association with viral vRNP. These findings indicate that hCLE, a cellular protein important for viral replication, is one of the very few examples of transcription factors that are incorporated into particles of an RNA-containing virus.

SUBMITTER: Rodriguez-Frandsen A

PROVIDER: S-EPMC4749964 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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hCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles.

Rodriguez-Frandsen Ariel A de Lucas Susana S Pérez-González Alicia A Pérez-Cidoncha Maite M Roldan-Gomendio Alejandro A Pazo Alejandra A Marcos-Villar Laura L Landeras-Bueno Sara S Ortín Juan J Nieto Amelia A

Scientific reports 20160211

The influenza A virus polymerase associates with a number of cellular transcription-related factors, including the RNA polymerase II (RNAP II). We previously described that the cellular protein hCLE/C14orf166 interacts with and stimulates influenza virus polymerase as well as RNAP II activities. Here we show that, despite the considerable cellular shut-off observed in infected cells, which includes RNAP II degradation, hCLE protein levels increase throughout infection in a virus replication-depe ...[more]

PMID: 26864902

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Dataset Information

HCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles.

Publications

hCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles.

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