HCLE/C14orf166, a cellular protein required for viral replication, is incorporated into influenza virus particles.

ABSTRACT: The influenza A virus polymerase associates with a number of cellular transcription-related factors, including the RNA polymerase II (RNAP II). We previously described that the cellular protein hCLE/C14orf166 interacts with and stimulates influenza virus polymerase as well as RNAP II activities. Here we show that, despite the considerable cellular shut-off observed in infected cells, which includes RNAP II degradation, hCLE protein levels increase throughout infection in a virus replication-dependent manner. Human and avian influenza viruses of various subtypes increase hCLE levels, but other RNA or DNA viruses do not. hCLE colocalises and interacts with viral ribonucleoproteins (vRNP) in the nucleus, as well as in the cytoplasm late in infection. Furthermore, biochemical analysis of purif

SUBMITTER: Rodriguez-Frandsen A 

PROVIDER: S-EPMC4749964 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature