Epigenetic Classification of Human Mesenchymal Stromal Cells.
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ABSTRACT:
SUBMITTER: de Almeida DC
PROVIDER: S-EPMC4750140 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Publications
Epigenetic Classification of Human Mesenchymal Stromal Cells.
de Almeida Danilo Candido DC Ferreira Marcelo R P MR Franzen Julia J Weidner Carola I CI Frobel Joana J Zenke Martin M Costa Ivan G IG Wagner Wolfgang W
Stem cell reports 20160201 2
Standardization of mesenchymal stromal cells (MSCs) is hampered by the lack of a precise definition for these cell preparations; for example, there are no molecular markers to discern MSCs and fibroblasts. In this study, we followed the hypothesis that specific DNA methylation (DNAm) patterns can assist classification of MSCs. We utilized 190 DNAm profiles to address the impact of tissue of origin, donor age, replicative senescence, and serum supplements on the epigenetic makeup. Based on this, ...[more]
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